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. 2014 Dec 15;211(13):2635–2649. doi: 10.1084/jem.20140470

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© 2014 Wang et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 1. — Tespa1 expression and BMMCs from WT and Tespa1-deficient mice. (a) Tespa1 mRNA expression in various cell subsets was measured by RT-PCR. Results are presented relative to Actb expression. Error bars represent SD. (b) BMMCs were stained with Alcian blue-Safranin O. Bars, 2 µm. (c) BMMCs were analyzed by electron microscopy. Bars, 2 µm. (d) Tissue mast cells in skin sections were stained with toluidine blue. Arrowheads indicate mast cells stained with toluidine blue. Bars: (top row) 100 µm; (bottom row) 25 µm. (e) Absolute numbers of mast cells per mm² (mean and SD). Data are representative of three experiments.