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. 2014 Dec 15;211(13):2635–2649. doi: 10.1084/jem.20140470

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© 2014 Wang et al.

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Figure 2. — FcεRI-mediated cytokine and chemokine production by Tespa1-deficient mast cells. (a) WT and Tespa1 KO BMMCs were sensitized with anti-DNP IgE overnight and later stimulated with DNP-BSA for 1, 3, or 24 h. Cytokine and chemokine mRNA expression was measured by RT-PCR. (b) IL-4, IL-5, IL-13, IL-6, TNF, and MCP-1levels were measured by ELISA at 24 h. *, P < 0.05; **, P < 0.01 (Student’s t test). Data are representative of three experiments. Error bars represent SD.