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. 2014 Dec 15;211(13):2617–2633. doi: 10.1084/jem.20140317

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© 2014 Di Foggia et al.

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Figure 7. — Increased ROS production and oxidative-stress induced DNA and protein damage in Bmi1^−/− cells is accompanied by MT1 down-regulation. (a) Protein analysis of MT1 and 3-NT levels in Bmi1^−/− muscle tissue and satellite cells cultures (representative results from three independent experiments with n = 3 for each condition). (b) The amount of ROS production is measured via DCFDA relative fluorescence intensity in Bmi1^−/− satellite cells compared with controls isolated from both SOL and EDL and cultured either in growth or differentiation conditions (mean ± SEM from two independent experiments with n = 4 for each condition; *, P < 0.05; **, P < 0.01; ***, P < 0.001). (c) Immunolabeling for 8-OHdG in Bmi1^−/− satellite cell cultures and controls (representative results from two independent experiments with n = 4 for each condition). (d) Quantification of 8-OHdG^+ve cells in Bmi1^−/− satellite cell cultures and controls in both growth and differentiation conditions (mean ± SD from two independent experiments with n = 4 for each condition; **, P < 0.01; ***, P < 0.001). Bar, 125 µm.