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. 2014 Dec 15;211(13):2617–2633. doi: 10.1084/jem.20140317

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© 2014 Di Foggia et al.

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Figure 9. — MT1 up-regulation is essential for the efficient contribution of Bmi1^Over satellite cells to muscle regeneration upon transplantation in the mdx mouse. (a) Satellite cells are isolated from mdx;STOPFloxBmi1 mice, cultured for 14 d, infected with A-Cre virus to activate Bmi1 expression, and treated either with Sh scramble and Mt1 knockdown, both viruses expressing GFP in infected cells. 6 d after treatment, the cells are transplanted in the TA muscles of mdx recipient mice. The transplanted muscles are collected after 27 d. Representative pictures of mdx muscles transplanted with A-Cre; mdx;STOPFloxBmi1 + Sh Scr satellite cells (a, left) or A-Cre; mdx;STOPFloxBmi1 + Sh MT1 satellite cells (a, right) are shown (a, representative results from n = 4 for each condition). (b) Quantification of GFP^+ve fibers in A-Cre; mdx;STOPFloxBmi1 + Sh MT1 transplanted satellite as compared with A-Cre; mdx;STOPFloxBmi1 + Sh MT1 transplanted satellite (mean ± SEM from one experiment with n = 4 A-Cre;Mdx;STOPFloxBmi1+Sh Scr and n = 4 Mt1 A-Cre;Mdx;STOPFloxBmi1+ShMt1; *, P < 0.05). Bar, 125 µm.