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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: RSC Adv. 2014 Oct 31;4(102):58762–58768. doi: 10.1039/C4RA11225C

Figure 1. Chemical syntheses and optical properties of 3 (ZW800-1C) and 4 (ZW800-3C).

Figure 1

Figure 1

(a) Synthesis of compounds 3 and 4 by the Suzuki-coupling using palladium catalyst in water. (b) LC-MS analysis and purity (%) of C-C coupled compounds: absorbance at 700 nm (top) and total ion chromatogram (TIC, bottom), and ESI-TOF mass spectrum (inserted). (c) Absorbance and fluorescence spectra of fluorophores 3 and 4 measured in PBS (pH 7.4) and FBS (supplemented with 50 mM HEPES, pH 7.4). (d) Optical properties of fluorophores 3 and 4 in various solvents (e) Molecular Brightness in 100% FBS supplemented with 50 mM HEPES, pH 7.4.