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Allelic mutations in the GmFAD3-2a gene identified in soybean mutant lines by TILLING. Boxes represent exons (white, open reading frames; gray, untranslated regions). Nucleotide and amino acid substitutions between the wild-type cultivar ‘Bay’ (top) and mutants (bottom) are indicated. Numbers indicate the distance from the first nucleotide of the start codon or from the initial methionine. Arrows indicate the positions of gene-specific primers for PCR reactions.
