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. 2014 Dec 1;64(4):371–377. doi: 10.1270/jsbbs.64.371

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Copyright © 2014 by JAPANESE SOCIETY OF BREEDING

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 6 — Functional analysis of wild-type (WT) and GmFAD3-2a mutant soybean genes expressed in yeast. Chromatograms of total fatty acids obtained from yeast cells harboring (pYES/CT) an empty vector or the same vector containing (WT) the ‘Bay’-derived GmFAD3-2a, (P1-A9) the mutant GmFAD3-2a containing a point mutation from ‘P1-A9’, (P1-A9Del) the mutant GmFAD3-2a containing a deletion in exon 8 from ‘P1-A9’, (P3-D1) the mutant GmFAD3-2a containing a point mutation from ‘P3-B1’, or (P3-F4) the mutant GmFAD3-2a containing a point mutation from ‘P3-F4’ by using flame ionization detector (FID). Arrowheads indicate α-linolenic acid.