Fig. 4.

In vivo interaction of FRG1 and SUVR2. (A) DNA blot showing that FLAG-tagged FRG1 complements the DNA methylation defect of frg1/2 at the MEA-ISR locus. Two biological replicates per genotype are shown. (B) Abundance of FRG1 and SUVR2 peptides in mass spectrometric analyses of four independent FRG1-FLAG IP experiments. The combined relative abundances of FRG1 and SUVR2 peptides are set to 100% on the x axis. (C) Co-IP assay for interaction of FRG1-FLAG with Myc-SUVR2. Input shows the expression of the tagged proteins in parental lines (P) and in the offspring from the crossed parental lines (F1). Myc-SUVR2 was immunopurified (IP) with anti-Myc antibody and the presence of copurified FRG1-FLAG was analyzed by Western blot using anti-FLAG antibody.