Fig. 4.

Stable cell lines expressing actin-sensor and actinin-sensor chimeras show normal cell physiology. We created 13 cell lines (Table S1). The MDCK and HEK stable cell lines were cultured in media in a 5% CO₂ chamber on a heated stage. A 20-h time lapse sequence from each cell line was used to monitor cell proliferation (BF, bright field; YFP, YFP channel signal from cpVenus). Using the Zeiss Definite Focus, we monitored at least five cell colonies simultaneously. Arrowheads indicate the dividing cells. All cells went through mitosis and proliferation. (Scale bar, 50 μm.)