. 2014 Nov 24;111(49):17420–17425. doi: 10.1073/pnas.1412743111

Table 1.

Apparent equilibrium dissociation constants

Parent transcript	RNA sequence: 123456789	D231V:WT affinity ratio
*HBB* intron1 (13-mer)	`UU CCCACCCUU AG`	2.2
HBB(U8 > A)	`UU CCCACCCAU AG`	0.4
*NF1* intron10a (13-mer)	`GU UUUGUUUUU AG`	2.3
NF1(U3 > A)	`GU UUAGUUUUU AG`	2.2
NF1(U8 > A)	`GU UUUGUUUAU AG`	0.3
NF1(U8 > G)	`GU UUUGUUUGU AG`	0.5
*NF1* intron10a (9-mer)	`UUUGUUUUU`	2.0
*RP2* intron3	`GUUUGCUUA`	2.5
RP2(U4 > A)	`GUUAGCUUA`	2.6
RP2(U8 > A)	`GUUUGCUAA`	1.0
*RP2* intron 4	`UAUUUAAAA`	0.7

Four nucleotides in the 5′ region and four nucleotides in the 3′ region are expected to contact RRM2 and RRM1, respectively, of U2AF⁶⁵. The anticipated D231V-bound nucleotide is in italic type. Nucleotide mutations relative to the WT (Wt) RNA sequence are highlighted in bold.