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. 2014 Aug 11;15:271. doi: 10.1186/1471-2474-15-271

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© Wang et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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ALP activity was increased during the osteogenic differentiation of AECs. (A) The ALP activity was detected by BCIP/NBT staining (magnification of 400×). (B) The percent of ALP-positive cells (containing blue, insoluble, granular dye deposit) was calculated by ImageJ software Experiments were performed three times with the similar results (n = 3 in each group). * indicates P < 0.05 vs Control, ^# indicates P < 0.05 vs PEMF and & indicates P < 0.05 vs OM.