Table 1.
Primer sequences and amplicon sizes for real-time RT-PCR
| Genes | Gene Bank ID | Primer Sequence (5'-3') | Amplicon (bp) |
|---|---|---|---|
| ALP | NM_000478 | F: 5' GGAACTCCTGACCCTTGACC 3' | 86 |
| R: 5' TCCTGTTCAGCTCGTACTGC 3' | |||
| OC | NM_001199662 | F:5' AGGGCAGCGAGGTAGTGAA 3' | 151 |
| R: 5' TCCTGAAAGCCGATGTGGT 3' | |||
| BMP-2 | NM_001200 | F:5' TCAAGCCAAACACAAACAGC 3' | 103 |
| R: 5' AGCCACAATCCAGTCATTCC 3' | |||
| RUNX2 | NM_001015051 | F:5' TTACTTACACCCCGCCAGTC 3' | 139 |
| R: 5' TATGGAGTGCTGCTGGTCTG 3' | |||
| β-catenin | NM_020248 | F:5' CCTATGCAGGGGTGGTCAAC 3' | 95 |
| R: 5' CGACCTGGAAAACGCCATCA 3' | |||
| Integrinβ1 | NM_002211 | F:5' CAAGCAGGGCCAAATTGTGG 3' | 121 |
| R: 5' TGTCATCTGGAGGGCAACCC 3' | |||
| Nrf2 | NM_001145413 | F:5' ATTGCCTGTAAGTCCTGGTCA 3' | 182 |
| R: 5' ACTGCTCTTTGGACATCATTTCG 3' | |||
| Keap1 | NM_203500 | F:5' GTGGCTGTCCTCAATCGTCT 3' | 127 |
| R: 5' GGATGGTGTTCATTGCTGTG 3' | |||
| β-actin | NM_001099771 | F:5' ACTATCGGCAATGAGCGGTTC 3' | 77 |
| R: 5' ATGCCACAGGATTCCATACCC 3' |