Inhibition of RPS15A expression in U2OS cells by RNAi. U2OS cells were successfully infected with Lv-shRPS15A(S1) (A) or Lv-shRPS15A(S2) (B), as detected by fluorescence microscopy (scale bar: 100 μm). Knockdown efficiency of RPS15A in U2OS cells by Lv-shRPS15A(S1) (C) or Lv-shRPS15A(S2) (D) determined by quantitative real-time polymerase chain reaction. Con, no lentivirus treatment; Lv-shCon, control lentivirus; Lv-shRPS15A, lentivirus containing an shRNA targeting RPS15A. **p<0.01, ***p<0.001. GFP, green fluorescent protein; RNAi, RNA interference.