Table 1.
Characterization of fosA3-carrying E. coli isolates.
| Isolatea | Farmb | Isolation date | PFGE patternc | MLST | Resistance profiled |
|---|---|---|---|---|---|
| AHC8 | F1 | 2011.6 | 1 | ST4472 | AMK, CTX, GEN, CIP, FFC, TET |
| AHC9 | F1 | 2011.6 | 2 | ST48 | CTX, GEN, CIP, TET |
| AHC12 | F1 | 2011.6 | 3 | ST533 | CTX, GEN, CIP, FFC, TET |
| AHC16 | F2 | 2011.6 | 3 | ST533 | CTX, GEN, CIP, FFC, TET |
| AHC19 | F4 | 2011.6 | 3 | ST533 | CTX, GEN, CIP, FFC, TET |
| AHC14 | F1 | 2011.6 | 4 | ST746 | CTX, CIP, FFC, TET |
| AHC17 | F2 | 2011.6 | 5 | ST4483 | AMK, CTX, GEN, CIP, FFC, TET |
| AHC18 | F3 | 2011.6 | 6 | ST4447 | CTX, CIP, TET |
| AHC23 | F5 | 2011.6 | 7 | ST2607 | AMK, CTX, GEN, CIP, FFC, TET |
| AHC24 | F5 | 2011.6 | 8 | ST155 | AMK, CTX, GEN, CIP, FFC, TET |
| AHC26 | F6 | 2011.6 | 9 | ST23 | AMK, CTX, GEN, CIP, TET |
| AHC27 | F6 | 2011.6 | 10 | ST2179 | CTX, GEN, CIP, FFC, TET |
| AHC30 | F6 | 2011.6 | 11 | ST226 | CTX, CIP, FFC, TET |
| AHC33 | F7 | 2011.6 | 12 | ST101 | AMK, CTX, GEN, CIP, FFC, TET |
| AHC51 | F9 | 2011.7 | 13 | ST57 | CTX, GEN, CIP, FFC, TET |
| AHC52 | F9 | 2011.7 | 14 | ST206 | AMK, CTX, GEN, CIP, FFC, TET |
| AHC76 | F8 | 2011.7 | 15 | ST4466 | AMK, CTX, GEN, CIP, FFC, TET |
| AHC54 | F9 | 2011.7 | 16 | ST155 | AMK, CTX, GEN, CIP, TET |
| AHC55 | F9 | 2011.7 | 17 | ST162 | CTX, GEN, CIP, FFC, TET |
| AHC57 | F10 | 2011.7 | 18 | ST10 | AMK, CTX, GEN, FFC, TET |
| AHC60 | F11 | 2011.7 | 19 | ST48 | AMK, GEN, CIP, FFC, TET |
| AHC66 | F12 | 2011.7 | 20 | ST48 | CTX, GEN, CIP, FFC, TET |
| AHC67 | F12 | 2011.7 | 21 | ST2223 | CTX, GEN, FFC, TET |
| AHC69 | F13 | 2011.7 | 22 | ST2847 | CTX, CIP, FFC, TET |
| AHC72 | F13 | 2011.7 | 23 | ST2847 | CTX, CIP, FFC, TET |
| AHC80 | F14 | 2011.7 | 24 | ST155 | AMK, CTX, GEN, CIP, FFC, TET |
| GDC27 | F15 | 2010.8 | 25 | ST156 | CTX, GEN, FFC, TET |
| GDC40 | F16 | 2010.8 | 26 | ST744 | CTX, GEN, CIP, FFC, TET |
| GDC54 | F18 | 2010.8 | 27b | ST2496 | CTX, GEN, CIP, FFC, TET |
| GDC46 | F17 | 2010.8 | 27a | ST4460 | CTX, GEN, CIP, FFC, TET |
| GDC47 | F17 | 2010.8 | 28 | ST746 | CTX, CIP, FFC, TET |
| GDC114* | F28 | 2010.9 | 29 | ST48 | AMK, CTX, GEN, CIP, FFC, TET |
| GDC56 | F19 | 2010.8 | 30a | ST359 | CTX, GEN, CIP, FFC, TET |
| GDC58 | F23 | 2010.8 | 30b | ST4461 | CTX, CIP, FFC, TET |
| GDC61 | F20 | 2010.8 | 31 | ST4473 | CTX, GEN, FFC, TET |
| GDC15 | F21 | 2010.8 | 32 | ST4465 | CTX, CIP, FFC |
| GDC16 | F21 | 2010.8 | 33 | ST4474 | AMK, CTX, GEN, CIP, FFC, TET |
| GDC17 | F21 | 2010.8 | 34 | ST1518 | CTX, CIP, FFC, TET |
| GDC24 | F15 | 2010.8 | 35 | ST4477 | CTX, CIP, FFC, TET |
| GDC37 | F22 | 2010.8 | 36 | ST4459 | CTX, GEN, FFC, TET |
| GXC03* | F24 | 2009.7 | 37 | ST2847 | CTX, GEN, CIP, FFC, TET |
| GXC19* | F25 | 2009.7 | 38 | ST4360 | CTX, GEN, CIP, FFC, TET |
| HNC02* | F31 | 2009.7 | 39 | ST4464 | AMK, CTX, GEN, FFC, TET |
| HNC06* | F31 | 2009.7 | 40 | ST779 | CTX, TET |
| SDC20 | F35 | 2009.6 | 41 | ST48 | CTX, FFC, TET |
| SDC10 | F32 | 2009.6 | 42 | ST4498 | AMK, CTX, GEN, CIP, FFC, TET |
| SDC12 | F32 | 2009.6 | 43 | ST4497 | AMK, CTX, GEN, CIP, FFC, TET |
| SDC04 | F33 | 2009.5 | 44 | ST602 | CTX, CIP, FFC, TET |
| SDC01 | F34 | 2009.5 | 45 | ST4462 | AMK, CTX, GEN, CIP, FFC, TET |
| SDC11 | F34 | 2009.6 | 46 | ST602 | AMK, CTX, GEN, CIP, TET |
| SDC15 | F36 | 2009.5 | 47 | ST219 | AMK, CTX, GEN, CIP, FFC, TET |
| GD326 | F27 | 2011.3 | 48 | ST1589 | CTX, CIP, FFC, TET |
| GDC1-4* | F28 | 2010.9 | 49 | ST453 | CTX, GEN, CIP, FFC, TET |
| GDC240* | F29 | 2010.8 | 50 | ST354 | AMK, CTX, GEN, CIP, FFC, TET |
| GDC540* | F29 | 2010.8 | 51 | ST48 | CTX, GEN, FFC, TET |
| GDC1-2* | F28 | 2010.9 | 52 | ST93 | CTX, GEN, CIP, FFC, TET |
| GDC127* | F30 | 2010.9 | smeared | ST156 | CTX, GEN, CIP, FFC, TET |
| SDC13 | F37 | 2009.5 | smeared | ST398 | CTX, GEN, CIP, FFC, TET |
Different provinces are indicated as follows: AH, Anhui; GD, Guangdong; GX, Guangxi; HN, Henan; SD, Shandong. Isolates from which the fosA3 gene can be transferred to the recipient by conjugation are underlined. Healthy animals are indicated by an asterisk.
F1 to F36, farm 1 to farm 36, respectively.
PFGE types (1, 2, 3, etc.) were assigned by visual inspection of the macrorestriction profile. Patterns that differed by fewer than six bands were considered to represent subtypes within the main group (30a, 30b, etc.). NT, nontypeable.
AMK, amikacin; CTX, cefotaxime; CIP, ciprofloxacin; FFC, florfenicol; GEN, gentamicin; TET, tetracycline. The antimicrobial susceptibility results were interpreted according to breakpoint of CLSI (M100-S23), except that florfenicol (≥32 μg/mL) was interpreted according to breakpoint of European Committee on Antimicrobial Susceptibility Testing (EUCAST). All isolates were susceptible to colistin, imipenem, piperacillin-tazobactam, and tigecycline. Resistance phenotypes transferred to the recipient by conjugation ortransformationare underlined.