Table 2.
Characterization of some plasmids carrying fosA3.
| Plasmid(s) | Co-transfer of other resistance gene(s) | Context of fosA3a | Plasmid | |||
|---|---|---|---|---|---|---|
| Size (kb) | Replicon type | EcoRI RFLP b | Addiction systems | |||
| GDC24, GDC58, AHC18, SDC13, GDC40, GDC47, GDC1-4, GDC114, SDC04 | blaCTX−M−55 | I | ~75 | F33:A-:B- | A1 | hok-sok, pemKI, srnBC |
| AHC33, GDC240 | blaCTX−M−65, rmtB | III | ~75 | F33:A-:B- | A1 | hok-sok, pemKI, srnBC |
| AHC17, AHC26 | blaCTX−M−55, rmtB | I | ~100 | N-F33:A-:B- | A2 | hok-sok, pemKI, srnBC, vagCD |
| AHC24 | blaCTX−M−55, rmtB, floR | I | ~110 | N-F33:A-:B- | A3 | hok-sok, pemKI, srnBC, vagCD |
| GDC54 | blaCTX−M−65 | III | ~75 | F33:A-:B- | A4 | hok-sok, pemKI, srnBC, vagCD |
| AHC23 | blaCTX−M−65, rmtB | III | ~80 | F33:A-:B- | A5 | hok-sok, pemKI, srnBC |
| GDC46 | blaCTX−M−55 | III | ~75 | F33:A-:B- | A6 | hok-sok, pemKI, srnBC, vagCD |
| SDC01 | blaCTX−M−55, rmtB | I | ~100 | N-F33:A-:B- | A7 | hok-sok, pemKI, srnBC, vagCD |
| AHC76 | blaCTX−M−55 | I | ~75 | F33:A-:B- | A8 | hok-sok, pemKI, srnBC |
| HNC02 | blaCTX−M−65, rmtB | III | ~80 | F33:A-:B- | A9 | hok-sok, pemKI, srnBC, vagCD |
| AHC52 | blaCTX−M−55, rmtB, floR | I | ~110 | N-F33:A-:B- | A10 | hok-sok, pemKI, srnBC, vagCD |
| GDC17 | blaCTX−M−55, floR | I | ~95 | N-F33:A-:B- | B | hok-sok, pemKI, srnBC, vagCD |
| AHC9 | blaCTX−M−55 | I | ~65 | N-F33:A-:B- | C | pemKI, srnBC, vagCD |
| AHC69, AHC72 | blaCTX−M−65 | I | ~75 | F33:A-:B- | ND | hok-sok, pemKI, srnBC, vagCD |
| AHC27 | blaCTX−M−65 | I | ~115 | I1/ST71 | D1 | pemKI, pndCA, vagCD |
| GDC27 | blaCTX−M−65, floR | I | ~125 | I1/ST71 | D2 | pemKI |
| AHC30 | blaCTX−M−55, floR | I | ~105 | I1/ST136 | D3 | hok-sok, pemKI, pndCA, vagCD |
| GXC19 | blaCTX−M−65, floR | II | ~125 | I1/ST71 | D4 | pndCA |
| AHC54 | blaCTX−M−123 | I | ~110 | I1/ST108 | D5 | pemKI, pndCA, vagCD |
| AHC14 | blaCTX−M−123 | I | ~115 | I1/ST108 | D6 | pndCA |
| AHC55 | blaCTX−M−123 | I | ~110 | I1/ST108 | D7 | hok-sok, pemKI, pndCA |
| SDC11 | blaCTX−M−14, rmtB | V | ~100 | I1/ST136 | D8 | pemKI, pndCA |
| AHC60 | rmtB | Unknown | ~120 | I1 | E | pndCA, vagCD |
| AHC66, AHC67 | blaCTX−M−65, floR | I | ~230 | HI2/ST3 | F1 | none |
| AHC57 | blaCTX−M−14, floR | IV | ~230 | HI2/ST3 | F2 | none |
| AHC80 | blaCTX−M−14, rmtB, floR | IV | ~230 | HI2/ST3 | ND | vagCD |
| GDC540 | blaCTX−M−14, floR | V | ~230 | HI2/ST3 | F3 | pemKI, vagCD |
| GDC15 | blaCTX−M−65, floR | III | ~230 | HI2/ST3 | F4 | none |
| GDC61 | blaCTX−M−14, floR | IV | ~230 | HI2 | F5 | pemKI, vagCD |
| GXC03 | blaCTX−M−15 | Unknown | ~230 | HI2/ST3 | F6 | vagCD |
| GDC127 | blaCTX−M−14, floR | V | ~230 | HI2/ST3 | F7 | none |
| HNC06 | blaCTX−M−14 | V | ~230 | HI2/ST3 | F8 | vagCD |
| GD326 | blaCTX−M−14, floR | V | ~230 | HI2 | F9 | none |
| SDC20 | blaCTX−M−65, floR | I | ~230 | HI2/ST3 | ND | none |
| SDC10, SDC12 | rmtB, floR | I | ~45 | N | ND | none |
| SDC15 | rmtB | I | ~50 | N | ND | vagCD |
| AHC51 | blaCTX−M−65 | I | ~50 | N | ND | none |
| GDC1-2 | blaCTX−M−3, floR | I | ~75 | F2:A-:B- | ND | hok-sok, pemKI, vagCD |
| GDC56 | blaCTX−M−3 | I | ~75 | F14:A-:B- | ND | hok-sok, pemKI, vagCD |
| AHC12 | blaCTX−M−65, floR | I | ~80 | F18:A-:B- | ND | pemKI, srnBC, vagCD, ccdAB |
| AHC16 | blaCTX−M−14, floR | I | ~80 | F18:A-:B- | ND | pemKI, srnBC, ccdAB |
| GDC16 | I | ~70 | unknown | ND | vagCD | |
a
Contexts of fosA3 were as follows: I, IS26-316bp-fosA3-1758bp-IS26; II, IS26-316bp-fosA3-536bp-IS26; III, blaCTX−M−14-1135bp-fosA3-1758bp-IS26; IV, blaCTX−M−14-611bp-fosA3-1222bp-IS26.
b
Restriction fragment length polymorphism (RFLP) patterns differed by only a few bands (n = 1–3) were assigned to the same RFLP profile. ND, not determined.