Table 2.
Experiment design for the effects of inoculation OD, nutrients, initial pH, pre-hydrolysis time, and temperature on the SSF performance of washed SECS and whole slurry by Saccharomyces cerevisiae
| Parameter | Effect of OD | Effect of nutrients | Effect of initial pH | Effect of pre-hydrolysis time | Effect of temperature |
|---|---|---|---|---|---|
| Initial OD600 | 1.0, 4.0, 8.0 | 4.0 | 4.0 | 4.0 | 4.0 |
| Nutrients | 50% | 0%, 50%, 100% | 50% | 50% | 50% |
| Initial pH | 4.8 | 4.8 | 4.0, 4.8, 5.5 | 4.8 | 4.8 |
| Pre-hydrolysis time (hours) | 36 | 36 | 36 | 24, 36, 48 | 36 |
| Temperature (°C) | 36 | 36 | 36 | 36 | 30, 33, 36, 39, 42 |
100% nutrients was defined as 10 g/L yeast extract and 20 g/L peptone; 50% nutrients was defined as 5 g/L yeast extract and 10 g/L peptone; 0% nutrients was defined as 0 g/L yeast extract and 0 g/L peptone. OD, optical density; SSF, simultaneous saccharification and fermentation; SECS, steam-exploded corn stover.