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Schematic view of the culture dish for irradiation. The dimensions are in cm and not at scale. a) The cell dish has 3.5 cm of diameter and depending on the experiment (A) or (B); approximately, 98 or 92% of this area was shielded by aluminum foils, respectively. Unshielded area was exposed to 5, 10, 50 and 100 mGy of α–particles emitted by a 210Po source. b) Schematic view of the bystander III-B area. Immediately after-irradiation the γ-H2AX assay was performed.
