FIGURE 2.

Nonadditive nature of pre- and postconditioning neuroprotection in the brain. (A) Schematic diagram of combined pre- and postconditioning. Animals were subject to 30 mins of MCAO, followed by 72 h reperfusion. Animals were then subject to 100 mins of MCAO, 10 mins of reperfusion, and a subsequent 10 mins of MCAO challenge. Infarct volume was determined 24 h later by TTC staining. (B) Quantification of pre-, postconditioning, and addition of pre- plus postconditioning ischemia. Note the lack of significant further protection by adding pre- and postconditioning. Data shown are mean7s.d. n=5. Data were analyzed by ANOVA with post hoc Bonferroni’s test, *P<0.01 versus 100 mins of MCAO. (From Pignataro et al. 2008)