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Primer sequences for amplification of C. difficile tcdA, tcdB, spo0A and 16S rRNA gene sequences
| Gene | Primer | Sequence (5′–3′) | Accession number |
|---|---|---|---|
| tcdA | tcdA-F | CAACACCTTAACCCAGCCATA | AM180355 |
| tcdA-R | AGAGTTTTCTGCGGTAGCTGA | AM180355 | |
| tcdB | tcdB-F | ATCTGGAGAATGGAAGGTGGT | AM180355 |
| tcdB-R | TGATGGTGCTGAAAAGAAGTG | AM180355 | |
| spo0a | spo0A-F | AGCGCAATAAATCTAGGAGCA | AM180355 |
| spo0A-R | AGGTTTTGGCTCAACTTGTGT | AM180355 | |
| 16S rRNA | 16S-F | AGCGGTGAAATGCGTAGATAT | AM180355 |
| 16S-R | CAG CGTCAGTTACAGTCCAGA | AM180355 |
