. 2014 Sep 24;70(1):116–123. doi: 10.1093/jac/dku358

Table 1.

Primer sequences and PCR cycling conditions for amplifying and sequencing full-length pfcrt

Gene	PCR primer sequence 5′ to 3′	Cycling conditions	Product size (bp)
pfcrt fragment 1 (exons 1–4)	outer PCR	94°C^{3 min}, (94°C^{1 min}; 45°C^{1 min}, 65°C^{1 min}) × 30 cycles, 65°C^{10 min}	873
	CRTP1: CCG TTA ATA ATA AAT ACA CGC AG
	NG1_REV: GGA TAC CAT AGC ATT TAA TC
	nested PCR	94°C^{3 min}, (94°C^{1 min}; 50°C^{1 min}, 65°C^{1 min}) × 30 cycles, 65°C^{10 min}	827
	CRTP1: CCG TTA ATA ATA AAT ACA CGC AG
	NG4_REV: AGC ATT TAA TCT TAA AAT GTC
pfcrt fragment 2 (exons 4–13)	outer PCR	94°C^{3 min}, (94°C^{1 min}; 50°C^{1 min}, 65°C^{1 min}) × 30 cycles, 65°C^{10 min}	874
	NG5_FOR: CTC GGA GCA GTT ATT ATT G
	NG3_Rev: ATT CCT TAT AAA GTG TAA TGC GA
	nested PCR	94°C^{3 min}, (94°C^{1 min}; 50°C^{1 min}, 65°C^{1 min}) × 30 cycles, 65°C^{10 min}	827
	NG2_FOR: GCT CTT GTA GAA ATG AAA TTA TC
	NG3_REV: ATT CCT TAT AAA GTG TAA TGC GA
5′ and 3′ UTR sequencing primers	NG6_REV: GAA CAT AAT CAT ACA AAT A
5′ and 3′ UTR sequencing primers	NG7_FOR: CCG TGT AGG AAA TAT TAT CT
Sequencing primers for confirming intron skipping	P11: TCCTTTTTCCAATTGTTCACTTC
	P13: GACGGAGCATGGGTAAGAAG
	P17: CAAATGGCTTGTTCGTTCATAA