Table 2.
Transcription Factor Expression Levels During 24h of Hyperosmotic Stress ± Enzyme Inhibition in mESC Monolayer Culture
| 24-h Inhibition | Oct4 | Nanog | Rex1 |
|---|---|---|---|
| Unstressed | 1.00±0.00 | 1.00±0.00 | 1.00±0.00 |
| Stress only (S) | 1.03±0.16 | 1.15±0.20 | 0.35±0.08a |
| PD98059 (MEK1)+S | 0.78±0.06 | 0.80±0.09 | 0.51±0.01a |
| U0126 (MEK1/2)+S | 0.27±0.03a,b | 0.67±0.32 | 0.31±0.12a |
| SB202190 (p38)+S | 0.51±0.07a,b | 0.10±0.01a,b | 0.19±0.07a |
| L-JNKi-1 (JNK)+S | 0.91±0.15 | 0.71±0.15 | 0.89±0.08b |
| LY294002 (PI3K)+S | 0.65±0.08 | 0.44±0.07a,b | 0.00a,b |
| AKT1+S | 0.84±0.19 | 0.97±0.03 | 0.44±0.04a |
Values represent percent of unstressed expression.
a
Means statistically different from unstressed.
b
Means statistically different from “stress only,” n≥3, ANOVA and Student-Newman-Keuls post hoc tests, P<0.05.