Mean (+SEM) density (spines per 10 μm) of filopodial (F), thin (T), stubby (S), mushroom (M) and multiheaded (X) spines on medium spiny neurons in the NAc core and shell for control (n = 8), 1 day withdrawal (n = 8) and 7 day withdrawal (n = 9) groups. * indicates a significant group effect for stubby spines in the core at p = 0.038 and contrasts [7 day > control, p = 0.043; 7 day > 1 day, p = 0.028]. ‡ indicates a significant group effect for mushroom spines at p = .046 and contrasts [1 day > control, p = 0.042; 7 day > 1 day, p = 0.04]. † indicates a contrast for stubby spines in the shell [7 day > control, p = 0.049; 7 day > 1 day, p = 0.023]. Only the latter contrast was significant following correction.