Figure 11.6.
Assessment of gene expression for bias in GC content. The GC% of detected biotypes is binned along the x-axis and compared to mean expression values of raw counts (top) and normalized expression by reads per kilobase per million (RPKM) mapped reads (bottom). Alcoholic and control samples follow similar trends in raw mean expression values (A) and RPKM values (C) in relation to GC%. Mean raw count values in controls and alcoholics are persistently correlated with % of GC content (B); however, GC% shows lower correlation with normalized expression (D).