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. 2014 Nov 20;42(22):13588–13598. doi: 10.1093/nar/gku1146

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Effect of EHF depletion on wound repair in Calu-3 cells. (A) Images of negative control (NC) and EHF siRNA treated cells at 3, 6, 9 and 12 h after wounding. The outline of the wound is traced with a gray line. (B) Depletion of EHF significantly reduced the rate of wound closure at 6 and 9 h after wounding (n = 3). This difference was no longer evident 12 h after wounding. **P<0.01, ***P<0.001. (C) siRNA knockdown of EHF was measured by western blot in lysates taken 27 h after wounding and the signal was quantified using densitometry. EHF depletion was maintained throughout the experiment.