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. 2014 Nov 20;42(22):13588–13598. doi: 10.1093/nar/gku1146

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Effect of EHF depletion on transepithelial resistance in Calu-3 cells. (A) TER measurements in a single experiment show that EHF depleted Calu-3 cells (EHF siRNA, gray) had higher TER values compared to negative control cells (NC siRNA, black) at 48 to 96 h post-transfection. (B) TER measurements EHF siRNA normalized to NC; EHF depletion resulted in a significantly higher TER than NC-treated cells at 48 (*P< 0.05), 72 (***P<0.001) and 96 h (****P<0.0001) (n = 3). (C.) Western blot shows NC and EHF siRNA transfected Calu-3 cells from a single TER experiment (in triplicate) lysed at 96 h and depletion of EHF protein relative to β-tubulin was confirmed.