Table 1. Binding affinities of the human FAAP20 UBZ–ubiquitin complexes measured by ITC.
FAAP20 UBZ | Ubiquitin | Kd (μM) |
---|---|---|
WT | WT | 9.26 |
WT | I44A | NDB |
D164A | WT | NDB |
A168Y | WT | NDB |
L171A | WT | 529 |
W180A | WT | NDB |
NDB: No detectable binding or too weak to fit reliably (Kd > 600 μM).