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. 2014 Nov 21;42(22):13812–13823. doi: 10.1093/nar/gku1170

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — SRSF1 and Tat bind overlapping sequences within TAR. (A) RAC assays were set up with bait RNAs containing the wild-type (TARWT) and mutated (TARM) TAR sequences. The RNA substrates were incubated with 100 ng of recombinant Tat or purified SRSF1 in separate reactions. (B) Tat and SRSF1 compete for binding onto TAR. RAC assays were set up with the wild-type TAR sequence as bait, and either 100 ng of recombinant Tat and increasing amounts of purified SRSF1 (upper panel) or increasing amounts of Tat and 100 ng of SRSF1 (lower panel).