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. 2014 Nov 20;42(22):13615–13632. doi: 10.1093/nar/gku1186

Figure 1.

Figure 1.

dnTCF1EWT and dnTCF1Emut doxycycline inducible system in DLD-1 colon cancer cells. (A) In the absence of doxycycline, dnTCF1E is not produced and high levels of β-catenin (red) signaling activate Wnt target gene expression. At 2 h of doxycycline induction, low amounts of dnTCF1EWT and dnTCF1Emut (green) are produced, which compete with endogenous LEF/TCF factors (orange) for binding sites and cause a downregulation of Wnt target gene expression. At 9 h post-induction, there are higher levels of dnTCF1E induced, which cause increased repression of Wnt target gene expression. (B) Immunofluorescence images of dnTCF1EWT and dnTCF1Emut expression in DLD-1 cells. dnTCF1EWT and dnTCF1Emut (green) are found exclusively in the nucleus (stained blue with DAPI). (C) Western blot analysis of a Doxycycline induction time course of FLAG-dnTCF1EWT and FLAG-dnTCF1Emut expression.