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. 2014 Nov 20;42(22):13861–13872. doi: 10.1093/nar/gku1208

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Effect of ratchet helix mutations on Mtr4 activity. (A) Poly(A) RNA unwinding rate (k_unw) constants determined for Mtr4^WT and Mtr4 mutants, plotted as a function of Mtr4 concentration. Best fit curves to the data were calculated as described in ‘Materials and Methods’. Data presented here represent averages from three independent experiments; error bars represent SD. (B) Enhancement of ATPase activity in the presence of poly(A) RNA for Mtr4^WT and ratchet helix mutants. Data presented here represent averages from two independent experiments; error bars represent SD. (C) RNA unwinding gels of a poly(A) tail for Mtr4^WT, Mtr4^{R1030A-archless} and Mtr4^{E1033A-archless} reveal no unwinding activity for the Mtr4^{R1030A-archless} and Mtr4^{E1033A-archless} double mutants.