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R.CglI nuclease activity. (A) Phage λ DNA cleavage by R.CglI. Reactions contained 0.01 μg/μl phage λ DNA, 200 nM R.CglI or N.CglI, 4 mM ATP, ADP or AMP-PNP (as indicated above the lanes) and were conducted as described in ‘Materials and Methods’ section. (B) Run-off sequencing to determine the cleavage position of R.CglI. The recognition sequence 5′-GCCGC-3′ is indicated by the rectangle whilst the cleavage sites are indicated by arrows.
