Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Nov 26;42(22):13949–13962. doi: 10.1093/nar/gku1247

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 6. — Conversion of the BLV pre-miRNA to a 5′-monophosphate does not precede Dicer processing. (A) 5′-end characterization of BLV-pre-miR-B4. Northern blot analysis was performed on small RNAs purified from either HEK293T or HEK293T NoDice cells transfected with the pBLV-B4 miRNA expression vector and treated with the indicated enzymes. (B) Graphical representation of the northern blot analysis band density ratio (Terminator+/Terminator−) of BLV-pre-miR-B4 and tRNA in either HEK293T cells (WT) and HEK293T NoDice cells (NoDice) relative to the 5S RNA. The bars represent the average ratio of three independent biological replicates +/− the standard deviation (n = 3).