Table 4. The effect of AS GYNNGY on protein sequences.

	Total	Change < 10 AAsb	Change >10 AAsb	Frameshift	Stop (%)c
Strongly regulateda	75	0 (0%)	1	74	54(72%)
Other regulateda	144	5 (3.5%)	3	136	107(74%)
Unregulateda	291	7 (2.4)	5	279	216(74%)

^aThe grouping of sites is as defined in the text, ‘strongly regulated’ sites have ΔUMS ≥ 0.25 and ‘other regulated’ consists of all other regulated sites.

^bThese two types of changes occur when the translational frame is recovered to the original one through AS at the downstream acceptor site (the number of changed amino acids (AAs) are recorded).

^cThe number of cases introducing stop codons in the immediate downstream exon. No difference exists between these three groups of sites (Fisher's exact test, all P-values > 0.1).