Figure 1.

Assessment of genome editing by sequence trace decomposition. (a) Due to imperfect repair after cutting by a targeted nuclease, the DNA in the cell pool consists of a mixture of indels, which yields a composite sequence trace after the break site. (b) Overview of TIDE algorithm and output, which consists of three main steps: (1) Visualization of aberrant sequence signal in control (black) and treated sample (green), the expected break site (vertical dotted line) and the region used for decomposition (gray bar); (2) Decomposition yielding the spectrum of indels and their frequencies; (3) Inference of the base composition of +1 insertions. See main text and http://tide.nki.nl for explanation.