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. 2014 Oct 27;42(22):e169. doi: 10.1093/nar/gku940

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Application of an optimal sequencing quality filter. Comparison between permissive (a maximum of three bases with quality value lower than Q18 are allowed) and restrictive (a maximum one base with quality value lower than Q20 is allowed) filtering parameters. (A) Example of peptides rescued by applying a less restrictive quality filter to the selection against PK. The rescued peptides with the highest abundance are indicated (top 10). (B) DNA sequence of rescued peptides. The homopolymers in the DNA sequences are underlined. (C) Effect on the number of reads passing the different filtering parameters.