Table 1. Summary of the protein targets and peptide phage display libraries.
Target | Librarya | Library diversityb | Phage inputc | Phage outputc | Ion Torrent reads | Total sequencesd | Different sequencese | % top 200f |
---|---|---|---|---|---|---|---|---|
SrtA | Library A | 5 × 108 | 3 × 1010 | 8 × 103 | 1.8 × 105 | 5.1 × 104 | 2.8 × 103 | 26% |
SrtA | Library B | 1 × 107 | 2 × 1010 | 1 × 104 | 2.4 × 105 | 6.8 × 104 | 1.4 × 103 | 75% |
uPA | Library B | 1 × 107 | 9 × 109 | 3 × 104 | 3.4 × 105 | 1.1 × 105 | 3.1 × 103 | 56% |
FXIIa | 4×4 | 7 × 108 | 5 × 1010 | 1 × 104 | 4.1 × 105 | 1.7 × 105 | 7.9 × 103 | 15% |
PK | 3×3, 4×4 | 1 × 109 | 2 × 109 | 2 × 103 | 1.8 × 105 | 7.5 × 104 | 1.4 × 103 | 40% |
SA | 3×3, 4×4 | 1 × 109 | 2 × 109 | 4 × 102 | 1.1 × 105 | 6.1 × 104 | 3.4 × 102 | 84% |
aLibraries are named according to reference (24). Library A contains 3×4, 4×3, 4×4, 3×5, 5×3 peptides, library B contains 3×6, 6×3, 4×5, 5×4 peptides.
bNumber of transformants.
cTransducing units (t.u.).
dTotal number of sequences after quality filter.
eEstimated number of different sequences.
fPercentage of the population corresponding to the top 200 clones.