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. 2014 Dec 16;9(12):e115180. doi: 10.1371/journal.pone.0115180

Figure 10. ApoD facilitates binding of HDL to growing T24 cells.

Figure 10

T24 cells grown in 24-well plates were washed with protein-free RPMI two times, and 200 µl/well RPMI supplemented with 0.1%BSA was added with or without HDL (total HDL, 200 ng/well) and with or without apoD (20 ng/well) or apoA1 (20 ng/well). After incubation for 1 hour at 21°C, the plate was washed three times with protein-free RPMI, and bound apoA1 was released using detergent and assayed by apoA1 ELISA. In A) the cells were non-confluent and in B) the cells were confluent. Means ± SD of four experiments are shown. ** p<0.01, ****p<0.0001; p-values were calculated using two-way ANOVA with Holm-Sidal multiple comparison test, GraphPad Prism 6.