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. 2014 Dec 16;9(12):e114617. doi: 10.1371/journal.pone.0114617

Figure 1. The aur2-1/+ mutant is trisomy 2.

Figure 1

(A) Rosette phenotype of wild type (Col-0) and progeny from self-fertilized aur2-1/+. W, wild-type rosette leaves; R, round-shaped rosette leaves; T, tiny rosette leaves. (B) Metaphase spreads of the mitotic cells from Col-0 and aur2-1/+. (C) Meiosis in pollen mother cells of Col-0 and aur2-1/+. DAPI-stained chromosomes in different meiotic stages. Yellow arrows, extra univalents; red arrow, pentavalent; yellow stars, extra sister chromatids. (D) Fluorescence in situ hybridization analysis of aur2-1/+. Metaphase spreads of the aur2-1/+ mitotic cells probed with FITC-labeled 45S rDNA (green) and rhodamine-labeled 5S rDNA (red). Dashed lines, outline of DAPI-stained chromosome. Scale bar in (A) 2 cm; (B) 5 µm; (C) 10 µm; (D) 2 µm.