Figure 1. Tyro3, Axl, and Mertk receptors are expressed in primary hippocampal NSCs.

(A) Examination of TAM receptors expression in hippocampal NSCs by western blotting. (B and C) NSC spheres were obtained and processed for immunohistochemistry following the procedures described in the Methods. The cryosections were immunostained with anti-Tyro3, -Axl or -Mertk antibodies, accordingly (B, red) and nuclei were counter-stained with Hoechst 33342 (blue). The NSC spheres from the TKO mice were used as the negative control (C). Scale bar, 100 µM representing all the scale in (B and C).