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. 2014 Dec 16;9(12):e115134. doi: 10.1371/journal.pone.0115134

Figure 2. Effect of rTp0965 on adherence of monocytes to HUVECs.

Figure 2

(A) Confluent HUVECs were stimulated with rTp0965 (800 ng/ml) for 24 h. Monocyte THP-1 cells stained with calcein AM were then added and incubated for 6 h. The percentage of THP-1 cells were counted under the fluorescence microscope. (B–E) Confluent HUVECs were incubated with various concentrations of the rTp0965 for increasing time intervals. The levels of ICAM-1 and E-selectin mRNA in HUVECs were assayed by RT-PCR. (F-I) Confluent HUVECs were incubated with various concentrations of rTp0965 for increasing time intervals. The expression levels of ICAM-1 and E-selectin were measured by cell ELISA. (* = P<0.05, compared to a control). Results shown are those of one experiment representative of three similar experiments.