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. 2014 Dec 16;9(12):e115134. doi: 10.1371/journal.pone.0115134

Figure 4. Effects of rTp0965 on the permeability of HUVEC monolayers. Confluent HUVECs in the wells were preincubated with rTp0965 (800 ng/ml) for 24 h.

Figure 4

(A). HRP was then added to the transwell inserts and the flux of HRP in supernatants was calculated at 1 and 4h. (B) Effects of rTp0965 on migration of monocytes across HUVECs. Confluent monolayers of HUVECs cultured in Transwell inserts were pretreated with Y-27632 for 30 mins and HUVECs were then stimulated with rTp0965 (800 ng/ml) for 24 h. Monocyte THP-1 cells stained with calcein AM were then added in the inserts and the percentage of THP-1 cells in the wells was counted at 4 h under the fluorescence microscope.