Skip to main content
. 2014 Dec 16;9(12):e115134. doi: 10.1371/journal.pone.0115134

Figure 5. Effects of rTp0965 on F-actin reorganization. Confluent HUVECs were left untreated (a) or were stimulated with rTp0965 (800 ng/ml; b) for 24 h.

Figure 5

(A). Other HUVECs were preincubated with Y-27632 for 30 mins and then stimulated with rTp0965 (800 ng/ml; c). Cells were then fixed and were stained with rhodamine-phalloidin. (B) Effect of rTp0965 on HUVECs expression of claudin-1. Confluent HUVECs were stimulated with rTp0965 (800 ng/ml) or preincubated with Y-27632 for 30 mins and then stimulated with rTp0965 (800 ng/ml) for 2 h. The expression of claudin-1 in total cell lysates were evaluated by Western blot. A representative of three experiments is shown.