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. 2014 Dec 16;9(12):e115249. doi: 10.1371/journal.pone.0115249

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© 2014 Huang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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HeyA8 cells were cultured as 3D spheroids with different number of cells per spheroid. (A) At 5000 cells per spheroid, there was a broad shift in CXCR4 surface expression. At higher cell numbers per spheroid, the expression levels of surface CXCR4 increased. (B) Corresponding normalized pERK levels showed a strong correlation between increasing CXCR4 surface expression with decreasing phospho-ERK levels. (*, P <0.05 vs Adh, 1-way ANOVA) (***, P <0.0001 vs Adh, 1-way ANOVA). (C) At 100,000 cells per spheroid, HeyA8 cells treated with SDF-1α and PMA showed activation of phospho-ERK levels. (**, P <0.005 vs Adh, 1-way ANOVA). Each bar graph is representative of 3 experiments.