Abstract
Nuclei isolated from rat liver, when incubated with methyl-labeled S-adenosylmethionine, incorporated label into 2′-O-methyladenosine, 2′-O-methylguanosine, 2′-O-methyluridine, and 2′-O-methylcytidine of endogenous nuclear RNA. Addition of ribosomal RNA from wheat germ to the reaction markedly stimulated 2′-O-methylation of total RNA. The relative incorporation of label into the four different 2′-O-methyl ribonucleosides was greatly changed by the addition of wheat germ RNA. There was much more 2′-O-methylation of the purine ribonucleosides, relative to the pyrimidine ribonucleosides, in the reaction stimulated by wheat germ RNA.
Keywords: S-adenosyl methionine, wheat-germ RNA, paper chromatography
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