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. Author manuscript; available in PMC: 2015 Jun 4.
Published in final edited form as: Nature. 2014 Nov 12;516(7529):112–115. doi: 10.1038/nature13961

Figure 2. Activation of PPARα or FXR controls autophagy in liver.

Figure 2

a and b, Fed or fasted WT PPARα−/−, or FXR−/− mice treated with Veh, GW7647 or GW4064. LC3 Immunoblots of liver samples. Lanes are pooled (n=5 per group). c, Representative confocal images (out of 9 tissues sections per condition) of liver GFP-LC3 puncta (green: autophagosomes), DAPI (blue: DNA). Fed or fasted GFP-LC3Tg/+, bigenic PPARα−/−; GFP-LC3Tg/+, or FXR−/−; GFP-LC3Tg/+ mice treated with Veh, GW7647, or GW4064. Fixed liver samples analyzed by confocal microscopy. Scale bar, 50 µm. GFP puncta per cell are quantified in graph. Data represent mean±s.e.m. of 9 tissue sections. (n=3 per group, **P < 0.01 vs Fed GFP-LC3Tg/+ mice treated with Veh). Statistics by two-tailed t-test.