Figure 2.
LRRKHA is expressed and functional. (A) Efficiency of the two heat-shock-induced endonuclease events (I-SceI and I-CreI) used to induce double-strand breaks, assessed by PCR using primers over the I-SceI site and in LRRK as well as using primers over I-CreI and in GluRIIE. When the I-SceI or I-CreI site is absent, no PCR product can be formed. (B) Schematic representation showing where primers anneal to generate PCR product over the introduced HA-tag and (B′) PCR products, the higher product indicates the presence of the triple HA tag, the lowest band indicates the product without the tag. (C) Western blot using HA antibody showing a 250-kDa large band of LRRKHA and using anti-synapsin antibody as a loading control. Right: Ponceau Red staining of the same blot.