Figure 4.

Engineering of the salm gene. (A) Step 1 engineering of the salm gene. The genomic salm organization is depicted with coding exons in orange. The sgRNA targeting sites are indicated by red arrows and the resulting salm[1^st exon-dsRed], [1^st intron-dsRed] and [3^rd exon-dsRed] alleles are shown. (B) Step 2 engineering of the salm gene. RMCE products of the salm[1^st intron-dsRed] with three different exon cassettes are shown.