Figure 5.

Phenotypic analysis of the engineered salm alleles. (A) Lethality assay of the salm-dsRed alleles as homozygous or in trans to salm[1]. (B) All tagged salm[1^st intron] alleles regain homozygous viability after step 2. (C–G) Localization of the tagged Salm proteins. Untagged Salm is located in the nucleus of wild-type IFMs (C, E), whereas V5 tagged Salm is only detected in the salm[1^st intron-TY1-V5] and the salm[1^st intron-FRT-TY1-FRT-V5] alleles (D, G). FLAG is found in the IFM nuclei of salm[1^st intron-GFP-FLAG] adults (F). The Salm-FRT-TY1-FRT-V5 protein is found in the nuclei (red arrow heads) and also located in dots in the cytoplasm (G). Note the normal fibrillar morphology of the myofibrils in all the homozygous salm[1^st intron] alleles (D, F, G). Actin was stained with phalloidin and the scale bars are 10 µm.