Table 1. Summary of the transformation efficiencies for the four different genomic locations modified in this study.
| Location | Injected Genotype | sgRNAs | Injected Embryos | Larvae | G0 Adults | Fertile G0 Adults | Independent G0 Founders | Total Red-Eyed F1 | Independent Founders Per Fertile G0s |
|---|---|---|---|---|---|---|---|---|---|
| salm[1st exon-dsRed] | Lig4−/− | sgRNA1 + sgRNA3 | 1500 | 396 | 166 | ND | 0 | 0 | ND |
| salm[1st intron-dsRed] | Lig4−/− | sgRNA3 | 700 | 171 | 149 | ND | 2 | 11 | ND |
| salm[3rd exon-dsRed] | Lig4−/− | sgRNA7 + sgRNA9 | 1500 | 312 | 150 | ND | 4 | 72 | ND |
| salm[1st exon-dsRed] | Act5C-Cas9, Lig4−/− | sgRNA1 + sgRNA3 | 700 | 124 | 48 | 30 | 1 | 13 | 3.3% |
| salm[1st intron-dsRed] | Act5C-Cas9, Lig4−/− | sgRNA3 + sgRNA4 | 700 | 200 | 122 | 64 | 9 | 54 | 14.1% |
| salm[3rd exon-dsRed] | Act5C-Cas9, Lig4−/− | sgRNA7 + sgRNA9 | 700 | 291 | 150 | 99 | 4 | 59 | 4.0% |
| bent | Act5C-Cas9, Lig4−/− | bent -sg1 + bent -sg3 | 700 | 204 | 118 | 56 | 1 | 5 | 1.8% |