Table 1. ShmIR constructs and summary of results.
| Construct | Length | Mismatches | AS Sequence | Knockdown Assayed by RT-PCR?; Result |
|---|---|---|---|---|
| sim-Lhr-shRNA-577 | 21 | Mismatch at bp 2 | TAGATTCATTGCTAACACCAT | Yes; knockdown |
| sim-msl-3-shRNA-579 | 21 | Mismatch at bp 2 | TATTGTGATAGAAGGTCTCGG | Yes; no knockdown |
| sim-msl-3-shRNA-599 | 21 | None | TAACATAGTTCTCCCTGTCGA | N/A; lethal to both sexes in D. melanogaster |
| sim-msl-3-shRNA-600 | 21 | None | TAGTACCTTGACCATATTCCG | Yes; lethal to D. melanogaster males |
| sim-msl-3-shRNA-601 | 21 | None | TAGCGCCGTCATCACTTGCAG | Yes; no knockdown |
| sim-msl-3-shRNA-633 | 22 | Mismatches at bp 2 and 11 | TGAATGGGACCAAGTTAGTCAC | N/A; lethal to both sexes in D. melanogaster |
| sim-msl-3-shRNA-634 | 22 | Mismatches at bp 2 and 11 | TCTCCCGTGTGGAGTGGATCCA | Yes; no knockdown. |
| sim-msl-3-shRNA-635 | 22 | Mismatches at bp 2 and 11 | TCGCACATGGGCATCGACCGAT | N/A; semilethal to both sexes in D. melanogaster |
ShmIR, short hairpin microRNA; AS, anti-sense; RT-PCR, reverse-transcription polymerase chain reaction; N/A, not applicable.