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. Author manuscript; available in PMC: 2015 Jun 11.
Published in final edited form as: Nature. 2014 Sep 28;516(7530):254–258. doi: 10.1038/nature13765

Extended Data Figure 9. P2RY8-dependent suppression of GC B cell survival and promotion of B cell confinement to the GC niche is receptor specific.

Extended Data Figure 9

(a) Transwell migration of WEHI231 cells transduced with retrovirus encoding the control Gα13-coupled receptor, Tbxa2r, toward CXCL12 (100 ng/mL) in the presence or absence of the thromboxane A2 analogue, U-46619. (b, c) Fold change in frequency of Thy1.1 reporter+ GC relative to Fo B cells of PPs (b) or mLN (c) from BM chimeras reconstituted with S1pr2 KO BM transduced with empty vector (control) or Tbxa2r. (d) Immunohistochemical analysis of splenic sections from SRBC-immunized mice given Gpr183+/- B cells transduced with empty vector, Tbxa2r or P2RY8, and assessed 24 h after cell transfer. Scale bar is 200 μm. Data in a and d are one experiment representative of 2. Data in b and c are from one experiment (n=4 in each group). **P<0.01, unpaired two-tailed Student's t test.